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In Vitro Models of Cutaneous Inflammation
Quentin Bernard1, Benoît Jaulhac2,3, Nathalie Boulanger4,5
1Department of Veterinary Medicine, College Park and Virginia-Maryland College of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2017
Summary
Understanding skin inflammation is crucial for fighting Lyme borreliosis. Studying resident skin cells in vitro can reveal how ticks and Borrelia burgdorferi sensu lato interact with hosts.
Area of Science:
- Dermatology
- Infectious Diseases
- Immunology
Background:
- The skin is the primary site of Borrelia burgdorferi sensu lato inoculation by Ixodes ticks, initiating Lyme borreliosis.
- Understanding the skin's inflammatory response is critical for developing effective Lyme disease diagnostics and vaccines.
Purpose of the Study:
- To explore the utility of in vitro skin cell cultures for studying host-pathogen interactions in Lyme borreliosis.
- To investigate the complex interplay between the tick, Borrelia burgdorferi sensu lato, and the host at the skin interface.
Main Methods:
- Utilizing in vitro cell culture models of resident skin cells.
- Analyzing the inflammatory reactions within these skin models upon exposure to tick components or Borrelia burgdorferi sensu lato.
Main Results:
- In vitro skin cell cultures provide a model to study the initial inflammatory events of Lyme borreliosis.
- This approach can help elucidate the mechanisms of pathogen transmission and host response at the inoculation site.
Conclusions:
- In vitro skin cell cultures are a valuable tool for deciphering the early stages of Lyme borreliosis.
- Further research using these models can inform the development of novel strategies against Lyme disease.