On-chip cell labelling and washing by capture and release using microfluidic trap arrays

Area of Science:

• Biotechnology
• Microfluidics
• Cell Biology

Background:

• Flow cytometry requires large, purified cell quantities for accurate analysis.
• Current methods for cell preparation are often time-consuming and labor-intensive.

Purpose of the Study:

• To develop a novel microfluidic device for efficient on-chip cell labelling, washing, and release.
• To enable the preparation of large cell numbers in a timely manner for flow cytometry.

Main Methods:

• Design and fabrication of a microfluidic trap structure array with streamlined shapes.
• Testing the device with leukocytes, varying flow speeds, incubation times, and release speeds.
• Demonstration of on-chip intracellular and extracellular cell labelling and washing using STYO13.

Main Results:

• The microfluidic device captures approximately 85% of cells, independent of input flow speed.
• Release efficiency exceeds 80% for captured cells with up to 20 minutes of incubation.
• The streamlined trap design minimizes cell clogging during capture and release.

Conclusions:

• The novel microfluidic trap array significantly enhances cell preparation efficiency for flow cytometry.
• The device facilitates on-chip labelling and purification, addressing the need for large cell quantities.
• Developed qualitative models provide design guidance for improved microfluidic cell preparation systems.