Influence of dental materials on cells of the equine periodontium
Related Experiment Video
Updated: Jul 22, 2026
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
H Ringeisen1, A Pöschke1, B Krähling1
1Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany.
Background:
Therapy for equine periodontal disease can include filling of the periodontal pockets and widened interproximal spaces. Recommended dental materials are generally adopted from human dentistry.
Objectives:
To evaluate the biocompatibility of dental materials for equine periodontal fillings in vitro.
Study Design:
In vitro experiments.
Methods:
Four different dental materials (PeriCare® , Provicol® , Calxyl® and Honigum) were tested on equine periodontal fibroblasts. Possible cytotoxic effects were assessed microscopically and by MTT assay, and the expression of inflammatory marker genes was measured by qRT-PCR.
Results:
PeriCare® and Provicol® had no effects on the cells, whereas Honigum and Calxyl® were associated with severe cytotoxic effects.
Main Limitations:
The results of this in vitro study need to be confirmed by clinical studies.
Conclusions:
Before adapting dental materials from human dentistry, it is crucial to initially test them in a specific equine model.
