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Related Experiment Video

Updated: Jul 6, 2026

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

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Published on: August 31, 2016

Endothelial transplantation rejuvenates aged hematopoietic stem cell function.

Michael G Poulos^1,2,3, Pradeep Ramalingam^1,2,3, Michael C Gutkin^1,2,3

¹Department of Medicine.

The Journal of Clinical Investigation

|October 17, 2017

Summary

Aging endothelial cells (ECs) in the bone marrow microenvironment impair hematopoietic stem cell (HSC) function. Young ECs can restore HSC function but not reverse myeloid bias, suggesting EC therapies for age-related hematopoietic decline.

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Intrafemoral Injection of Human Hematopoietic Stem and Progenitor Cells into Immunocompromised Mice

Intrafemoral Injection of Human Hematopoietic Stem and Progenitor Cells into Immunocompromised Mice

Published on: December 8, 2023

Area of Science:

Hematology
Gerontology
Stem Cell Biology

Background:

Age-related hematopoietic decline is often attributed to intrinsic hematopoietic stem cell (HSC) changes.
The role of the aged bone marrow (BM) microenvironment, particularly endothelial cells (ECs), in supporting HSC function remains under-evaluated.

Purpose of the Study:

To investigate the contribution of aged endothelial cells (ECs) within the bone marrow (BM) microenvironment to hematopoietic aging.
To assess the therapeutic potential of young ECs in restoring aged HSC function and improving hematopoietic recovery.

Main Methods:

Utilized an ex vivo hematopoietic stem and progenitor cell/EC (HSPC/EC) coculture system.
Administered young and aged EC infusions in vivo following myelosuppressive injury in mouse models.
Evaluated HSC repopulating activity, myeloid bias, and hematopoietic recovery post-treatment.

Main Results:

Aging of ECs was sufficient to induce hematopoietic aging phenotypes in young HSCs.
Aged ECs impaired young HSC repopulating activity and promoted a myeloid bias.
Young ECs partially restored aged HSC function but could not reverse the intrinsic myeloid bias.
Infusion of young ECs enhanced hematopoietic recovery and HSC function in aged mice, improving survival post-transplant.

Conclusions:

The BM microenvironment, specifically ECs, significantly contributes to age-related hematopoietic decline.
Young ECs hold therapeutic potential for enhancing HSC engraftment and accelerating hematopoietic recovery in elderly individuals.
EC-targeted therapies may serve as an adjuvant strategy to improve outcomes following myelosuppressive regimens in older populations.