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Impact of pH on Urine Chemistry Assayed on Roche Analyzers
Clinical Laboratory
|October 17, 2017
Summary
Urine pH affects some test results, but creatinine normalization eliminates this impact for clinical assessments. Measuring urine pH is still important for analytical and accreditation purposes.
Area of Science:
- Clinical chemistry
- Urine analysis
- Analytical toxicology
Background:
- Urine pH can influence the concentration of various urinary analytes.
- This influence raises questions about the necessity of pre-treating urine samples based on pH.
Purpose of the Study:
- To investigate the effect of pH on urine chemistry parameters using Roche Modular analyzers.
- To determine if analyte concentrations in patient urines are pH-dependent without pre-treatment.
Main Methods:
- In vitro analysis of urine chemistry parameters across different pH levels.
- Evaluation of patient urine samples, assessing analyte concentration in relation to native pH.
Main Results:
- Optimal pH ranges were identified for amylase, calcium, magnesium, phosphorus, and uric acid.
- Creatinine, sodium, and urea concentrations were unaffected by pH.
- While some parameters showed pH dependence in patient samples, creatinine normalization removed these associations.
Conclusions:
- Urine pH adjustment is not essential for evaluating renal function.
- Measuring urine pH is recommended for analytical and accreditation requirements due to its relationship with certain analytes.