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Related Experiment Videos

How does solvation in the cell affect protein folding and binding?

Caitlin M Davis1, Martin Gruebele2, Shahar Sukenik3

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Current Opinion in Structural Biology
|October 17, 2017
PubMed
Summary

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Cellular protein function is sensitive to the surrounding environment. New in situ studies reveal how changes in the cellular solvation environment, affecting protein surface area and volume, impact protein interactions and cellular processes.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Proteins are sensitive to their solute environment, but studies were rarely conducted in situ.
  • Live-cell monitoring techniques now allow protein studies in their natural cellular context.
  • The cellular solvation environment is increasingly recognized as crucial for protein function.

Purpose of the Study:

  • To explain how protein surface area and volume changes influence steric and non-steric interactions within the cell.
  • To highlight the impact of the cellular solvation environment on protein function.
  • To provide examples of how environmental changes affect cellular processes.

Main Methods:

  • In situ monitoring of proteins within live cells.
  • Analysis of protein surface area and volume changes.

Related Experiment Videos

  • Investigation of steric and non-steric interactions.

    • Main Results:

    • Protein surface area and volume changes are key regulators of interactions within the cellular environment.
    • The cellular solvation environment significantly affects protein function.
    • Mild environmental changes can lead to substantial alterations in cellular processes.

    Conclusions:

    • Understanding the cellular solvation environment is critical for comprehending protein function.
    • Protein surface area and volume are important factors in cellular processes.
    • In situ studies provide novel insights into protein behavior within the complex cellular milieu.