Related Experiment Video
Updated: Aug 5, 2026

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
Published on: July 25, 2013
Rational design of disordered proteins for sequence-function investigation
Kara Hunter1,2, Trevor Brandt1, Karina Guadalupe2
1Department of Chemistry, Syracuse University, Syracuse, NY, USA.
Abstract:
Despite lacking a stable three-dimensional structure, intrinsically disordered protein regions (IDRs) are ubiquitous across all kingdoms of life and have essential cellular roles1. While rational design of folded proteins has seen substantial recent progress2, our ability to design IDRs remains more limited3. Here we present GOOSE (Generate disOrdered prOteins Specifying propErties), a comprehensive computational framework for the rational design of IDRs. GOOSE's versatility and throughput enable us to design and test thousands of IDR sequences to reveal distinct sequence-to-function relationships. Using GOOSE to explore these relationships, we examine how sequence properties influence IDR structural ensembles in cells, design IDRs that respond to structural changes associated with cell volume decrease, create scaffold IDRs that self-assemble and recruit specific clients, and design novel IDRs that protect cells from desiccation. Our work uses rational sequence design as a powerful method for exploring function in IDRs and provides a versatile tool for designing functional disordered proteins.
More Related Videos
05:08Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
Published on: July 8, 2025
07:08Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
Published on: July 14, 2015
Related Concept Videos
Intrinsically Disordered Proteins
Protein Folding
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Protein Organization
The primary structure of a protein is its amino acid sequence.
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...