Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elemental Analysis in Biological Matrices Using ICP-MS.

Matthew N Hansen1, Jeffrey D Clogston2

  • 1Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mastering particle size analysis: lessons, challenges, and future directions from the FDA-CRCG workshop.

AAPS open·2026
Same author

Advancing Medical Applications of Cancer Nanotechnology: Highlighting Two Decades of the NCI'S Nanotechnology Characterization Laboratory Service to the Research Community.

Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology·2025
Same author

Cellular fate of a plant virus immunotherapy candidate.

Communications biology·2024
Same author

Current state of nanomedicine drug products: An industry perspective.

Journal of pharmaceutical sciences·2024
Same author

Nano-mupirocin as tumor-targeted antibiotic: Physicochemical, immunotoxicological and pharmacokinetic characterization, and effect on gut microbiome.

Journal of controlled release : official journal of the Controlled Release Society·2024
Same author

Advancements in Nanoparticle Characterization.

Methods in molecular biology (Clifton, N.J.)·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Tracking metallic nanoparticles in cancer therapy requires sensitive methods. Inductively coupled plasma mass spectrometry (ICP-MS) offers a precise way to analyze nanoparticle distribution in biological samples.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Metallic nanoparticles are increasingly explored for cancer therapeutics.
  • Tracking their distribution and clearance in vivo is crucial for safety and efficacy.
  • Existing methods may lack the sensitivity or specificity required.

Purpose of the Study:

  • To present a standardized method for analyzing metallic nanoparticle distribution in biological matrices.
  • To ensure complete tissue homogenization and proper preparation of standards and controls.
  • To provide a reliable technique for tracking nanotherapeutics in living systems.

Main Methods:

  • Utilized Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for sensitive metal analysis.
  • Developed a standardized protocol for processing biological samples, including tissue homogenization.
Keywords:
BiodistributionBiological matricesBloodHomogenizationICP-MSNanoparticles

Related Experiment Videos

  • Established procedures for preparing appropriate analytical standards and controls.

    • Main Results:

    • Demonstrated the effectiveness of ICP-MS for tracking gold nanoparticles in biological samples.
    • Validated a method for complete tissue homogenization, crucial for accurate quantification.
    • Outlined the preparation of standards and controls essential for reliable data.

    Conclusions:

    • ICP-MS is a sensitive and selective technique for tracking metallic nanoparticles in biological systems.
    • The presented standardized method facilitates accurate analysis of nanotherapeutic distribution.
    • This approach is adaptable for analyzing various metallic nanoparticles beyond gold.