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Related Experiment Videos

Analysis of Complement Activation by Nanoparticles.

Barry W Neun1, Anna N Ilinskaya1, Marina A Dobrovolskaia2

  • 1Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2017
PubMed
Summary

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Complement Activation Related Pseudo Allergy (CARPA) is a serious condition mediated by the complement system, not IgE. This study introduces a quantitative assay to assess CARPA risk from drug formulations.

Area of Science:

  • Immunology
  • Nanotechnology

Background:

  • The complement system aids innate immunity and pathogen clearance.
  • Complement Activation Related Pseudo Allergy (CARPA) mimics anaphylaxis but involves complement, not IgE.
  • CARPA is linked to certain drugs and nanotechnology products.

Purpose of the Study:

  • To present a quantitative enzyme-linked immunoassay (ELISA) for assessing complement activation.
  • To compare the ELISA method with a previous qualitative western blotting technique.
  • To provide updated details on nanoparticle sample preparation for assays.

Main Methods:

  • Utilized enzyme-linked immunoassay (ELISA) for quantitative analysis of complement activation.
  • Employed serum or plasma from healthy donors for in vitro assays.
Keywords:
AnaphylaxisC3ComplementEIAImmunoassayNanoparticlesWestern blot

Related Experiment Videos

  • Included revised protocols for nanoparticle sample preparation.

    • Main Results:

    • The ELISA method provides quantitative data on complement activation.
    • Performance comparison between ELISA and western blotting was conducted.
    • The study details nanoparticle preparation for improved assay accuracy.

    Conclusions:

    • In vitro complement activation assays are valuable for predicting CARPA risk.
    • The quantitative ELISA offers a robust method for CARPA assessment.
    • Updated methods enhance the evaluation of drug formulations for complement-mediated reactions.