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Untargeted Screening of Urinary Peptides Using Offline Nano-Liquid Chromatography: MALDI-TOF/TOF Mass Spectrometry
François-Ludovic Sauvage1,2, Sébastien Passeron1,2, Pierre Marquet3,4
1Department of Pharmacology-Toxicology-Pharmacovigilance, INSERM UMR 850, CHU Limoges, University of Limoges, 2 Avenue Martin-Luther King, Limoges Cedex, 87042, France.
Abstract:
In renal transplantation, the discovery of early urine biomarkers of graft lesions would be useful in helping physicians to improve patient care and minimize the use of invasive techniques such as biopsies. Over the last years, high-resolution mass spectrometry has been used extensively for the search of biomarkers in various biological fluids. Here we describe a procedure based on reverse-phase nano-HPLC, offline plate spotting, and MALDI-TOF and TOF/TOF applied in our laboratory for the search of natural peptides in urine samples from renal transplant patients.
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