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Related Experiment Videos

Spectroscopic Doppler analysis for visible-light optical coherence tomography.

Xiao Shu1, Wenzhong Liu2, Lian Duan1

  • 1Northwestern University, Department of Biomedical Engineering, Evanston, Illinois, United States.

Journal of Biomedical Optics
|October 19, 2017
PubMed
Summary

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This study introduces a new spectroscopic Doppler analysis method to measure retinal blood flow using visible-light optical coherence tomography (vis-OCT). This technique overcomes phase ambiguity limitations, enabling more accurate retinal blood flow quantification in vivo.

Area of Science:

  • Ophthalmology
  • Biomedical Optics
  • Medical Imaging

Background:

  • Retinal oxygen metabolic rate is crucial for eye health.
  • Visible-light optical coherence tomography (vis-OCT) measures oxygen saturation and blood flow.
  • Doppler OCT faces limitations in measuring unambiguous velocity due to phase ambiguity, especially with shorter wavelengths used in vis-OCT.

Purpose of the Study:

  • To develop and validate a novel spectroscopic Doppler analysis method to overcome phase ambiguity in vis-OCT.
  • To enable accurate quantification of retinal blood flow without the need for phase unwrapping.
  • To assess the feasibility of this method for in vivo retinal blood flow measurements.

Main Methods:

  • Spectroscopic Doppler analysis was employed by splitting the vis-OCT spectrum into narrow subbands.
Keywords:
optical Doppler tomographyoptical coherence tomographyphase variationspectroscopic analysis

Related Experiment Videos

  • vis-OCT images were reconstructed for each subband to extract Doppler phase shifts.
  • Subband-dependent phase shifts were analyzed using linear regression to quantify flow velocity.
  • Phantom experiments and in vivo rodent studies were conducted.

    • Main Results:

    • The spectroscopic Doppler analysis successfully extended the measurable absolute phase shift range.
    • The method eliminated the requirement for phase unwrapping, overcoming a key limitation of traditional Doppler OCT.
    • Accurate quantification of retinal blood flow in rodents in vivo was demonstrated.

    Conclusions:

    • Spectroscopic Doppler analysis is a robust method for enhancing vis-OCT capabilities.
    • This technique provides a more accurate and reliable way to measure retinal blood flow.
    • The developed method holds significant potential for clinical applications in ophthalmology.