Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

5.2K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
5.2K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

10.9K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
10.9K
Enzyme Kinetics01:19

Enzyme Kinetics

104.6K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.6K
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

34.2K
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
34.2K
Induced-fit Model01:13

Induced-fit Model

89.9K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
89.9K
Enzymes02:34

Enzymes

95.8K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
95.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Interstellar Mapping And Acceleration Probe High Energy (IMAP-Hi) Neutral Atom Imager.

Space science reviews·2026
Same author

The Solar Wind Electron (SWE) Instrument for the Interstellar Mapping and Acceleration Probe Mission.

Space science reviews·2026
Same author

Unveiling the enzymatic pathway of UMG-SP2 urethanase: insights into polyurethane degradation at the atomic level.

Chemical science·2025
Same author

Atomistic adsorption of PETase onto large-scale PET 3D-models that mimic reality.

Physical chemistry chemical physics : PCCP·2025
Same author

Sub-Bandgap Sensitization of Perovskite Semiconductors via Colloidal Quantum Dots Incorporation.

Nanomaterials (Basel, Switzerland)·2023
Same author

Inactivation Mechanism of the Fatty Acid Amide Hydrolase Inhibitor BIA 10-2474.

Chembiochem : a European journal of chemical biology·2022

Related Experiment Video

Updated: Feb 20, 2026

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

908

Protocol for Computational Enzymatic Reactivity Based on Geometry Optimisation.

N M F S A Cerqueira1, P A Fernandes1, M J Ramos1

  • 1REQUIMTE-UCIBIO, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|October 19, 2017
PubMed
Summary
This summary is machine-generated.

Computational enzymology uses advanced methods to reveal enzyme mechanisms. This approach details reaction pathways, intermediates, and energetics, offering insights beyond traditional experiments.

Keywords:
density functional calculationsenzyme catalysisenzyme modelsmolecular modellingreaction mechanisms

More Related Videos

Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

11.6K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.4K

Related Experiment Videos

Last Updated: Feb 20, 2026

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

908
Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

11.6K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.4K

Area of Science:

  • Biochemistry
  • Computational Chemistry
  • Enzymology

Background:

  • Enzymes are crucial for biological chemical processes.
  • Understanding enzyme mechanisms is complex and challenging.
  • Computational enzymology offers detailed mechanistic insights.

Purpose of the Study:

  • To present a general computational strategy for studying enzymatic mechanisms.
  • To provide an introduction to computational enzymology for non-specialized users.
  • To illustrate the power of computational methods with examples.

Main Methods:

  • Adiabatic mapping and free geometry optimization are key computational techniques.
  • High theoretical levels are employed for studying chemical reactions.
  • Model systems are utilized to investigate enzymatic reactions.

Main Results:

  • Computational methods elucidate complete enzymatic reaction mechanisms.
  • Detailed characterization of reaction intermediates and transition states is achieved.
  • The strategy allows for exhaustive exploration of the chemical reaction space.

Conclusions:

  • Computational enzymology provides unparalleled detail in studying enzyme catalysis.
  • The presented strategy enhances understanding of enzyme function.
  • This approach complements experimental validation and advances the field.