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Antitumor activity of yulangsan polysacchrides in mice bearing S180 sarcoma tumors
Wen'E Cai1, Xiaoyu Chen2, Qingdong Pan1
1Department of Clinical Pharmacy, 303 Hospital of PLA, Nanning, Guangxi 530021, P.R. China.
Abstract:
Sarcoma is one of the most prevalent pediatric tumors and the therapeutic role of chemotherapy has yet to be elucidated. It has been reported that extracts of Longyanshen (Yulangsan) may enhance the sensitivity of drug-resistant cancer cell lines, and improve the immune dysfunction induced by cyclophosphamide (CTX) in mice. The present in vivo study investigated the antitumor effects of Yulangsan polysaccharides (YLSPS) and their interaction with CTX in murine sarcoma 180 (S180)-bearing mice. Immunohistochemistry was used to detect the expression of apoptosis-related proteins. The ultrastructure of sarcoma cells was examined by transmission electron microscopy and the tumor growth rate was determined by measuring the tumor weight. A dose-dependent inhibition of sarcoma growth was observed in S180-bearing mice following administration of YLSPS. In combination with CTX, an additive antitumor effect was obtained, which was accompanied by amelioration of immune function. YLSPS also potentiated the tumor suppression effect of CTX while avoiding cytotoxicity against immune cells. YLSPS inhibited sarcoma growth in S180-bearing mice through the induction of apoptosis in S180 sarcoma cells. YLSPS also attenuated CTX-induced cytotoxicity to the immune system while potentiating the tumor suppression effect. These results provide additional information regarding combination therapy with YLSPS and chemotherapy for the treatment of sarcoma.
Insights
Yulangsan polysaccharides (YLSPS) show antitumor effects against sarcoma by inducing apoptosis. Combined with cyclophosphamide (CTX), YLSPS enhance chemotherapy
Area of Science:
- Oncology
- Immunology
- Pharmacology
Background:
- Sarcoma is a common pediatric tumor with an incompletely understood chemotherapy role.
- Longyanshen (Yulangsan) extracts may improve drug resistance and cyclophosphamide (CTX)-induced immune dysfunction.
Purpose of the Study:
- To investigate the antitumor effects of Yulangsan polysaccharides (YLSPS) in sarcoma.
- To evaluate the interaction between YLSPS and CTX in sarcoma treatment.
- To assess the impact on immune function and apoptosis.
Main Methods:
- In vivo study using murine sarcoma 180 (S180)-bearing mice.
- Immunohistochemistry to detect apoptosis-related proteins.
- Transmission electron microscopy for cell ultrastructure analysis.
- Tumor weight measurement to determine growth rate.
Main Results:
- YLSPS demonstrated dose-dependent inhibition of sarcoma growth.
- Combination therapy with YLSPS and CTX showed additive antitumor effects.
- YLSPS ameliorated immune dysfunction and potentiated CTX's tumor suppression.
- YLSPS induced apoptosis in S180 sarcoma cells and reduced CTX-induced immune cell cytotoxicity.
Conclusions:
- YLSPS exhibit significant antitumor activity against sarcoma.
- Combination of YLSPS with CTX offers a promising therapeutic strategy for sarcoma.
- YLSPS can enhance chemotherapy efficacy while mitigating immune-related side effects.
