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Updated: Feb 20, 2026

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
Published on: April 9, 2021
Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave
Jonas Lohse1, Alexandra Schindl, Natasha Danda
1Chemical Biology II, Stratingh Institute for Chemistry, University of Groningen, 9747AG Groningen, The Netherlands. m.d.witte@rug.nl.
Abstract:
A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.
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