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Proteus vulgaris and Proteus mirabilis Decrease Candida albicans Biofilm Formation by Suppressing Morphological
Kyoung Ho Lee1, Su Jung Park1, Sun Ju Choi1
1Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Korea.
Yonsei Medical Journal
|October 20, 2017
Summary
Proteus vulgaris and Proteus mirabilis inhibit Candida albicans biofilm formation by altering gene expression. Secretory products from Proteus species prevent the yeast-to-hyphal transition, a key virulence factor.
Area of Science:
- Microbiology
- Mycology
- Bacterial Pathogenesis
Background:
- Candida albicans and Proteus species are opportunistic pathogens causing nosocomial infections.
- Biofilm formation is a significant virulence factor for C. albicans.
Purpose of the Study:
- To investigate the effect of co-cultivation with Proteus vulgaris and Proteus mirabilis on C. albicans biofilm formation.
- To elucidate the underlying molecular mechanisms of this interaction.
Main Methods:
- Biofilm formation was quantified using XTT reduction assays.
- Gene expression of C. albicans was analyzed using real-time RT-PCR, focusing on yeast-specific, filament-inhibiting, and hyphae-related genes.
Main Results:
- Co-cultivation with live or heat-killed Proteus species, or Proteus-cultured supernatant, significantly inhibited C. albicans biofilm formation.
- Proteus species decreased the expression of hyphae-related genes while increasing yeast-specific and filament formation inhibiting genes in C. albicans.
Conclusions:
- Secretory products from Proteus vulgaris and Proteus mirabilis inhibit C. albicans biofilm formation.
- These products regulate C. albicans gene expression, suppressing the yeast-to-hyphal transition.
