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Genome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n = 50).

John L Williams1,2, Daniela Iamartino3,2, Kim D Pruitt4

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Summary

A new draft genome sequence for river water buffalo was assembled and annotated. This resource is crucial for understanding water buffalo biology and advancing breeding techniques.

Keywords:
Water buffaloannotationgenome assemblytranscriptome

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Area of Science:

  • Genomics
  • Animal Science
  • Bioinformatics

Background:

  • Water buffalo are vital for global agriculture and economies.
  • A comprehensive reference genome is needed for water buffalo research and breeding.
  • Previous genome assemblies for water buffalo were unavailable.

Purpose of the Study:

  • To create a de novo assembled, well-annotated reference genome sequence for the river water buffalo.
  • To facilitate studies on water buffalo biology, genetic diversity, and genomic selection.

Main Methods:

  • Utilized Illumina GA and Roche 454 short-read sequencing data.
  • Employed the MaSuRCA assembler for genome assembly.
  • Annotated the genome using transcriptome data from 30 tissues.

Main Results:

  • Generated a 2.83 Gb draft genome assembly with 366,983 scaffolds (N50 1.41 Mb).
  • Identified 21,711 predicted protein-coding genes.
  • Achieved 98.6% completeness for mammalian BUSCO gene groups, indicating high genome quality.

Conclusions:

  • The first draft reference genome sequence for river water buffalo has been successfully created and annotated.
  • This genome resource is publicly available and will support future research and breeding advancements in water buffalo.
  • The high completeness suggests a robust foundation for genomic studies in this important livestock species.