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Comparative study of GABA-T from glial cells, neuronal perikarya cells and synaptosomes
M P Bedoya1, C Toledo, M de Vicente
1Facultad de Farmacia, Instituto de Bioquímica (Centro mixto C.S.I.C.-U.C.M.), Madrid, Spain.
Abstract:
1. This paper presents a fast method of brain cell separation and a comparative study of GABA-T from different cellular compartments (glial cells, neuronal perikarya cells and synaptosomes). 2. The method of cellular separation offers the advantages of rapidity, ease and reproducibility. 3. The GABA-T from the three studied compartments had similar kinetic characteristics in respect of their Kms and Vmaxs. 4. The GABA-T needs PLP to reach its maximum activity; this dependence is related to the enzyme localization.