Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.2K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.2K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.7K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.7K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.4K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.4K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional genome reorganization enables cytokinin activation of NODULE INCEPTION during symbiotic nodulation.

Plant communications·2026
Same author

Replication-independent eviction of the histone variant H2B.8 reveals chromatin reprogramming during seed imbibition.

Nature communications·2026
Same author

<i>Arabidopsis</i> YEATS domain proteins facilitate DNA double-strand break repair via homology-directed pathways.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The I gene defines a dynamic NLR cluster conferring broad potyvirus resistance in common bean.

Nature communications·2026
Same author

Role of the tomato MARS1/ROUGH gene encoding a LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 in adventitious root and fruit skin formation.

Journal of integrative plant biology·2026
Same author

Thermo-sensitive tasiRNA biogenesis integrates temperature signals to maintain spikelet patterning in rice.

The New phytologist·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 20, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.0K

Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming.

Clara Bourbousse1, Moussa Benhamed2, Fredy Barneche3

  • 1Département de Biologie, IBENS, Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, 46 rue d'Ulm, F-75005, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2017
PubMed
Summary
This summary is machine-generated.

Chromatin dynamics are crucial for plant development and adaptation. New sensitive methods now allow detailed study of chromatin in specific plant cells, advancing molecular genetics and epigenomics.

Keywords:
ChromatinDNA methylationEpigenomeHistoneMethodology

More Related Videos

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.9K
Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines
11:39

Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines

Published on: June 17, 2017

18.9K

Related Experiment Videos

Last Updated: Feb 20, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.0K
A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.9K
Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines
11:39

Chromatin Immunoprecipitation ChIP in Mouse T-cell Lines

Published on: June 17, 2017

18.9K

Area of Science:

  • Plant molecular genetics and epigenomics.
  • Eco-evolutionary biology.

Background:

  • Chromatin landscape dynamics regulate plant development and adaptive responses.
  • Epigenetic inheritance of transcriptional contexts can occur.
  • Technical challenges previously limited chromatin profiling in specific plant cell types.

Purpose of the Study:

  • To introduce current methodologies for probing genome-wide chromatin variations.
  • To provide detailed protocols for analyzing chromatin in plants, focusing on Arabidopsis.

Main Methods:

  • Advances in genome-enabled technologies for increased sensitivity.
  • Improved methods for isolating specific plant cell types.
  • Techniques to probe nucleosome accessibility, composition, higher-order chromatin organization, and genome topology.

Main Results:

  • Overcoming limitations in studying chromatin in complex or inaccessible plant cell populations.
  • Revealing multilevel regulatory events influencing gene expression.
  • Highlighting the impact of chromatin dynamics on plant gene regulation.

Conclusions:

  • Uncovering the influence of chromatin dynamics and epigenetic processes is revolutionizing plant molecular genetics and epigenomics.
  • These advancements offer new perspectives for eco-evolutionary biology.
  • Methodologies presented enable deeper understanding of plant (epi)genomic regulation.