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Updated: Feb 20, 2026
Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
Published on: September 9, 2021
A novel upconversion luminescence derived photoelectrochemical immunoassay: ultrasensitive detection to
Xu Chen1, Wen Xu, Yandong Jiang
1State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, P. R. China. songhw@jlu.edu.cn.
Abstract:
Fabrication of near-infrared light-triggered photoelectrochemical (PEC) sensors based on the upconversion nanophosphors (UCNPs) is a novel approach, which exhibits the advantages of low background signal and non-damage to the biological substance as well as high sensitivity and improved electric detection in PEC sensors. Herein we demonstrate the preparation of novel and high-quality ZnO inverse opal photonic crystals (IOPCs)/Ag/NaYF4:Yb,Tm hybrid films by different advanced film techniques, including colloidal self-assembling, vapor phase deposition and pulsed laser deposition and its application to sensitive detection of alpha-fetoprotein (AFP). In the complex device, ZnO IOPCs and surface plasmon resonance (SPR) of silver had ability to largely enhance local excitation electromagnetic field of NaYF4:Yb,Tm, resulting in efficient near-infrared to visible/ultraviolet upconversion luminescence (UCL). The ultraviolet light emitted by NaYF4:Yb,Tm could b further reabsorbed by ZnO, generating PEC responses. Furthermore, because of the high specific surface area of ZnO IOPCs and the conductivity of Ag films, ZnO IOPCs/Ag/NaYF4:Yb,Tm hybrid films based near-infrared light-triggered PEC sensors showed ultrasensitive detection of AFP with a linear range from 0.05 ng mL-1 to 100 ng mL-1 and a low detection limit of ∼0.04 ng mL-1 (40 pg mL-1). Such an advanced device also shows promise in detection of other cancer markers in clinical and biological analysis.
