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Sandwich NP-based biobarcode assay for quantification C-reactive protein in plasma samples
Marta Broto1, Roger Galve1, M-Pilar Marco1
1Nanobiotechnology for Diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jordi Girona 18-26, 08034 Barcelona, Spain.
This study introduces a novel nanoparticle-based biobarcode assay for quantifying C-reactive protein (CRP) in plasma. The assay demonstrates high accuracy and potential for monitoring cardiovascular disease risk and inflammatory conditions.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Clinical Diagnostics
Background:
- C-reactive protein (CRP) is a key biomarker for inflammation and cardiovascular disease (CVD) risk.
- Accurate and sensitive quantification of CRP is crucial for patient monitoring and diagnosis.
Purpose of the Study:
- To develop and validate a novel nanoparticle-based biobarcode assay for C-reactive protein (CRP) quantification in plasma.
- To assess the assay's performance and its potential clinical utility in monitoring inflammatory conditions and CVD risk.
Main Methods:
- Utilized magnetic beads functionalized with capture antibodies (pAbCRP2-MP) to isolate target CRP from plasma.
- Employed multifunctional oligonucleotide-encoded probes with detection antibodies (pAbCRP1-ePSP) to form sandwich immunocomplexes.
- Integrated a fluorescent DNA microarray for detecting and quantifying released oligonucleotide codes.
Main Results:
- The NP-based biobarcode assay accurately quantified CRP in plasma samples within the range of 900-12500 ng/mL.
- Achieved excellent accuracy with a mean recovery of 99.5% ± 4.2%.
- Demonstrated utility in a pilot clinical study, correlating results with a reference method for CVD and inflammatory disease monitoring.
Conclusions:
- The developed NP-based biobarcode assay offers a sensitive and accurate method for CRP quantification.
- This assay shows promise for clinical applications in monitoring patients at risk of cardiovascular diseases and other inflammatory processes.
