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A High-Throughput Platform for Culture and 3D Imaging of Organoids
Published on: October 14, 2022
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Next generation organoids for biomedical research and applications
1Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Biotechnology Advances
|October 24, 2017
Summary
Organoid technology aims to create functional miniature organs for medical use. Reverse engineering principles and advanced biomaterials are key to overcoming challenges in cellular diversity and maturation for better disease modeling and drug discovery.
Area of Science:
- Biotechnology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Organoids are in vitro cultures mimicking organ structures from fetal or adult tissues.
- They hold immense potential for tissue replacement, disease modeling, toxicology, and drug discovery.
- Current limitations include insufficient cellular heterogeneity, inadequate microenvironment mimicry, and poor in vitro maturation.
Purpose of the Study:
- To address key challenges in human organoid technology.
- To advocate for reverse engineering principles to enhance organoid functionality and maturation.
- To propose a standardized pipeline for developing physiologically relevant human organoids.
Main Methods:
- Review of organoid development using fetal, adult, and pluripotent stem cells.
- Focus on studies utilizing self-organizing embryonic progenitors.
- Exploration of designer matrices, cell-free scaffolds, and adult stem cell niche factors.
Main Results:
- Highlighting the role of self-organization and engineered environments in organoid formation.
- Examining the interplay between adult stem cells and niche factors for maturation.
- Identifying key studies demonstrating progress in organoid technology.
Conclusions:
- Reverse engineering offers a pathway to overcome organoid limitations.
- Standardized protocols are crucial for generating more relevant human organoids.
- Advancements in organoid technology will significantly impact biomedical applications.
