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Published on: August 15, 2017
A cryptophane-based "turn-on" 129Xe NMR biosensor for monitoring calmodulin
Brittany A Riggle1, Mara L Greenberg, Yanfei Wang
1Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, USA. ivandmo@sas.upenn.edu.
Abstract:
We present the first cryptophane-based "turn-on" 129Xe NMR biosensor, employing a peptide-functionalized cryptophane to monitor the activation of calmodulin (CaM) protein in solution. In the absence of CaM binding, interaction between the peptide and cryptophane completely suppresses the hyperpolarized 129Xe-cryptophane NMR signal. Biosensor binding to Ca2+-activated CaM produces the expected 129Xe-cryptophane NMR signal.

