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Tocopherol levels in different mango varieties correlate with MiHPPD expression and its over-expression elevates
Rajesh K Singh1,2, Akhilesh K Chaurasia3, Rupesh Bari3
1Plant Gene Expression Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
Abstract:
Mango fruit tocopherol levels vary in different varieties during ripening. This study shows that tocopherol accumulation is highly correlated with its p-hydroxyphenyl pyruvate dioxygenase (MiHPPD) gene expression during ripening. MiHPPD transcript is ethylene induced and differentially expressed in four mango varieties used in this study. Higher/lower accumulation of tocopherol (mainly α-tocopherol) was achieved by heterologous expression of MiHPPD in Arabidopsis and tomato. The results suggest that tocopherol accumulation in mango fruit is correlated to MiHPPD gene expression. Over-expression of MiHPPD gene channelizes the flux towards tocophreol biosynthesis and could be used as a potential tool for metabolic engineering.
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