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Microarray expression profile analysis of long non-coding RNAs in optineurin E50K mutant transgenic mice.
Yuanyuan Li1, Lin Jin1, Aimeng Dong1
1Department of Ophthalmology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Molecular Medicine Reports
|October 26, 2017
Summary
Long non-coding RNAs (lncRNAs) are differentially expressed in the retinas of mice with the optineurin (OPTN) E50K mutation, suggesting their role in primary open angle glaucoma (POAG) pathogenesis.
Area of Science:
- Genomics
- Molecular Biology
- Ophthalmology
Background:
- Long non-coding RNAs (lncRNAs) play critical roles in cellular functions and disease development.
- Mutations in the optineurin (OPTN) gene, specifically the E50K substitution, are linked to primary open angle glaucoma (POAG).
Purpose of the Study:
- To identify lncRNAs associated with the OPTN (E50K) mutation in a mouse model.
- To investigate the functional implications of these lncRNAs in POAG pathogenesis.
Main Methods:
- Retinal tissue from OPTN (E50K) transgenic and wild-type mice was analyzed using lncRNA expression profiling via microarray.
- lncRNA and mRNA co-expression networks were constructed using Pearson's correlation analysis.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
Main Results:
- A total of 69 differentially expressed lncRNAs were identified (37 downregulated, 32 upregulated) between transgenic and wild-type mice.
- Coexpressed lncRNAs and mRNAs were significantly enriched in mRNA surveillance and RNA transport pathways.
- Two specific lncRNAs, ASMM10P055228 and ASMM10P040128, were linked to the regulation of oxidative stress-induced cell death and apoptosis.
Conclusions:
- lncRNA expression profiles are altered in the retinas of mice carrying the OPTN (E50K) mutation.
- These differentially expressed lncRNAs may contribute to the development of POAG associated with the OPTN (E50K) mutation.
