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Updated: Feb 20, 2026

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

RNA Flow Cytometry Using the Branched DNA Technique.

Kah Teong Soh¹, Paul K Wallace²

¹Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY, 14263, USA. kahteong.soh@roswellpark.org.

Methods in Molecular Biology (Clifton, N.J.)

|October 27, 2017

Summary

This summary is machine-generated.

This study introduces Branched DNA, a novel flow cytometry method for single-cell mRNA detection. It enables simultaneous measurement of mRNA and protein, overcoming limitations of bulk methods.

Keywords:

Branched DNA CD8 protein and mRNA correlation In-situ hybridization Peripheral blood mononuclear cells RNA flow cytometry

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Published on: April 28, 2021

Area of Science:

Molecular Biology
Cell Biology
Immunology

Background:

Cellular functions rely on precise mRNA and protein regulation.
Traditional methods like RNA-Seq are bulk analyses, lacking single-cell resolution.
Single-cell analysis is crucial for understanding cellular heterogeneity.

Purpose of the Study:

To introduce and detail the Branched DNA method for single-cell mRNA quantification.
To demonstrate the simultaneous detection of mRNA and protein using flow cytometry.
To correlate mRNA and protein expression at the single-cell level.

Main Methods:

Utilized Branched DNA technology, a flow cytometry-based platform.
Adapted the system for simultaneous detection of mRNA and protein using antibodies and fluorochromes.
Performed experiments on a model system to analyze CD8 mRNA and CD8 protein expression.

Main Results:

Branched DNA enables single-cell mRNA detection with high specificity and sensitivity.
The method allows for simultaneous quantification of mRNA and protein.
Successful correlation of CD8 mRNA and CD8 protein expression was achieved.

Conclusions:

Branched DNA is a powerful tool for single-cell gene expression analysis.
This method overcomes limitations of bulk analysis and provides deeper biological insights.
It offers an alternative for protein analysis when antibodies are unavailable or epitopes are inaccessible.