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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

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Multiparameter Conventional Flow Cytometry.

Katherine M McKinnon1

  • 1Vaccine Branch Flow Cytometry Facility, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 41, Room B715, 9000 Rockville Pike, Bethesda, MD, 20892, USA. mckinnonkm@mail.nih.gov.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2017
PubMed
Summary
This summary is machine-generated.

Multicolor flow cytometry enables simultaneous identification of multiple cell types using fluorescent markers. Successful implementation of advanced 12-color panels requires a systematic approach, not simple "plug and play" methods.

Keywords:
Flow cytometryImmunologyMulti-parameterMulticolor flow cytometryStaining

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Multicolor flow cytometry is crucial for analyzing complex cell populations in human and animal samples.
  • Simultaneous use of multiple fluorescent markers aids in identifying diverse cell types and their functions.
  • Advancements in instrumentation and reagents have increased the popularity and complexity of multicolor flow cytometry.

Purpose of the Study:

  • To provide a systematic approach for designing and implementing multicolor flow cytometry staining panels.
  • To address the challenges associated with adapting from lower to higher color panels (e.g., 4-6 colors to 12+ colors).
  • To guide researchers in achieving successful multi-parameter staining.

Main Methods:

  • Examination of considerations for multicolor flow cytometry panel design.
  • Discussion of systematic methods for multi-parameter staining.
  • Focus on adapting existing staining panels to higher color counts.

Main Results:

  • Successful multicolor flow cytometry requires careful planning and a systematic approach.
  • Transitioning to higher color panels (12+) presents challenges beyond simple "plug and play" adoption.
  • A structured methodology is essential for optimal results.

Conclusions:

  • Multicolor flow cytometry is a powerful tool for cell analysis.
  • Systematic panel design and execution are critical for high-parameter flow cytometry success.
  • Researchers must adopt methodical strategies to overcome the complexities of advanced multicolor flow cytometry.