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An aptasensor based on heparin-mimicking hyperbranched polyester with anti-biofouling interface for sensitive
Yanlian Niu1, Meilin Chu1, Ping Xu1
1National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Biosensors & Bioelectronics
|October 27, 2017
Summary
Researchers developed novel heparin-mimicking nanoparticles for an electrochemical biosensor. This aptasensor accurately detects thrombin in whole blood, showing potential for clinical diagnostics.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Thrombosis remains a significant clinical challenge, necessitating sensitive and specific detection methods for key biomarkers like thrombin.
- Developing advanced nanomaterials with anticoagulant properties is crucial for improving hemostatic devices and diagnostic tools.
- Electrochemical biosensors offer a promising platform for rapid and sensitive detection of disease markers.
Purpose of the Study:
- To synthesize novel heparin-mimicking hyperbranched polyester nanoparticles (HBPE-SO3 NPs) with sulfonated functional groups.
- To develop a label-free electrochemical aptasensor using HBPE-SO3 NPs for sensitive thrombin detection in whole blood.
- To evaluate the antithrombogenicity and anti-biofouling properties of the developed nanomaterials and biosensor.
Main Methods:
- Synthesis of HBPE-SO3 NPs and characterization of their properties.
- Fabrication of an electrochemical aptasensor by modifying an electrode with HBPE-SO3 NPs.
- Selection and utilization of a thrombin-binding aptamer as the recognition element.
- Electrochemical measurements to assess sensor performance, including detection range, limit of detection, selectivity, stability, and reproducibility.
- In vitro anti-biofouling tests using whole blood, platelet adhesion assays, and hemolysis tests.
Main Results:
- Successful synthesis of HBPE-SO3 NPs with abundant sulfonated acid groups, exhibiting heparin-mimicking properties.
- Development of a label-free electrochemical aptasensor demonstrating excellent sensitivity and selectivity for thrombin detection.
- Achieved a low detection limit of 0.031 pM (S/N = 3) for thrombin in whole blood.
- Demonstrated superior anti-biofouling capabilities compared to other modified electrodes.
- Validated the aptasensor's performance in detecting thrombin directly in whole blood samples.
Conclusions:
- The novel HBPE-SO3 NPs possess significant antithrombogenic and anti-biofouling properties.
- The developed electrochemical aptasensor offers a highly sensitive, selective, and stable platform for direct thrombin detection in whole blood.
- This aptasensor holds great potential for clinical applications in diagnosing and monitoring thrombotic disorders.
