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Updated: Feb 20, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
[Genome editing ~Principle and possibility of a novel genetic engineering technology. Basic principles of genome
1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Japan.
Abstract:
Programmable site-specific nuclease mediated-genome editing is an emerging biotechnology for precise manipulation of target genes. In genome editing, gene-knockout as well as gene-knockin are possible in various organisms and cultured cells. CRISPR-Cas9, which was developed in 2012, is a convenient and efficient programmable site-specific nuclease and the use spreads around the world rapidly. For this, it is important for the progress of life science research to introduce the genome editing technology.
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