Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-Platform Assessment of Sub-50 nm Nanopipette Emitters for Native Electrospray Ionization Mass Spectrometry.

bioRxiv : the preprint server for biology·2026
Same author

From Congestion to Clarity: On the Complementarity of Resolving Power and Spectral Simplification for Intact Protein Characterization.

Journal of the American Society for Mass Spectrometry·2026
Same author

On the Utility of Infrared Photoactivation for Native Top-Down and Complex-Down Orbitrap Mass Spectrometry of Soluble Proteoform Complexes.

Journal of the American Society for Mass Spectrometry·2026
Same author

Native Mass Spectrometry-Based Proteomics Reveals the Mechanism of Hemophore Release by Pathogenic <i>Corynebacterium diphtheriae</i>.

bioRxiv : the preprint server for biology·2026
Same author

Extensive Backbone Cleavage Coverage of Intact Proteoforms in a Mass Range of 10-70 kDa by Integrating Electron, Collision, and Photon-Based Fragmentation Techniques during an Electrophoretic Time Scale.

Journal of the American Society for Mass Spectrometry·2026
Same author

SPAP: Soluble Human Plasma Proteoform Analysis via Acetonitrile Precipitation and Top-Down Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2025
Same journal

Antimicrobial peptides from arthropod venoms exhibit activity against <i>Sporothrix</i> species.

The journal of venomous animals and toxins including tropical diseases·2026
Same journal

Distinct pathophysiological mechanisms of <i>Heterometrus laoticus</i> and <i>Lychas mucronatus</i> scorpion venoms on cardiovascular and renal functions.

The journal of venomous animals and toxins including tropical diseases·2026
Same journal

Cerebral <i>Aspergillus penicillioides</i> infection mimicking schwannoma in an immunocompetent host: case report with a review of the literature.

The journal of venomous animals and toxins including tropical diseases·2026
Same journal

A proactive multidisciplinary approach to <i>Naja atra</i> envenomation reduces surgical burden and length of hospital stay.

The journal of venomous animals and toxins including tropical diseases·2026
Same journal

Predictive value of admission blood glucose for early severity stratification in pediatric scorpion envenomation.

The journal of venomous animals and toxins including tropical diseases·2026
Same journal

A venom fraction from the Philippine tarantula (<i>Orphnaecus</i> sp.) reveals low-molecular-weight compounds that potentiate drug-like neurobehavioral responses in <i>Danio rerio</i>.

The journal of venomous animals and toxins including tropical diseases·2026
See all related articles

Related Experiment Video

Updated: Feb 20, 2026

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses
10:25

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses

Published on: November 3, 2014

34.6K

It is time for top-down venomics.

Rafael D Melani1, Fabio C S Nogueira1, Gilberto B Domont1

  • 1Proteomics Unit, Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149, CT A-542, Cidade Universitária, Rio de Janeiro, RJ CEP 21941-909 Brazil.

The Journal of Venomous Animals and Toxins Including Tropical Diseases
|October 28, 2017
PubMed
Summary
This summary is machine-generated.

Top-down proteomics (TDP) distinguishes toxin proteoforms (toxiforms) in animal venoms, overcoming limitations of bottom-up methods. This advanced technique enables detailed characterization of venom components and aids biotherapeutic development.

Keywords:
Denaturing top-down proteomicsNative top-down proteomicsTop-down proteomicsToxiformsVenomics

More Related Videos

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
12:16

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

24.9K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.1K

Related Experiment Videos

Last Updated: Feb 20, 2026

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses
10:25

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses

Published on: November 3, 2014

34.6K
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
12:16

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

24.9K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.1K

Area of Science:

  • Proteomics
  • Toxinology
  • Biochemistry

Background:

  • Animal venom composition is typically studied using bottom-up proteomics.
  • Bottom-up proteomics struggles to differentiate toxin proteoforms (toxiforms) due to the protein inference problem.

Purpose of the Study:

  • To highlight the advantages of Top-Down Proteomics (TDP) for characterizing venom toxiforms.
  • To introduce native and denaturing TDP as powerful tools in toxinology.

Main Methods:

  • Utilizing denaturing Top-Down Proteomics (TDP) for high-throughput analysis of proteoforms up to 30 kDa.
  • Employing native Top-Down Proteomics (TDP) to study large proteins (>50 kDa) and protein interactions.

Main Results:

  • TDP enables the identification and characterization of individual toxin proteoforms (toxiforms).
  • Native and denaturing TDP provide unprecedented detail on venom protein complexes at the toxiform level.

Conclusions:

  • Top-down venomics offers novel techniques for comprehensive venom analysis.
  • This approach deepens understanding of venom evolution and facilitates biotherapeutic development.