Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines
Divya S Vinjamur1, Daniel E Bauer2,3
1Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2017
Abstract:
The recently established human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines have equipped red blood cell researchers with valuable in vitro models of erythroid development. Of the three established HUDEP cell lines, HUDEP-2 cells express predominantly adult β-globin and most closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed.
Keywords:
CRISPR/Cas9Erythroid differentiationFetal hemoglobinHUDEPHemoglobin switchingImmortalized erythroid cell line
