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Homeostasis maintenance of encapsulated cells
Volha Liaudanskaya1,2, Claudio Migliaresi1,3, Antonella Motta1,3
1Department of Industrial Engineering, BIOtech Research Center, University of Trento, Trento, Italy.
Journal of Tissue Engineering and Regenerative Medicine
|October 28, 2017
Summary
Stabilizing engineered tissue homeostasis is crucial for transplantation. SHSY5Y neuroblastoma cells encapsulated for 28 days met transplantation requirements, demonstrating the importance of maturation time.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Cell niche homeostasis is vital for tissue function, stem cell maintenance, wound healing, and cancer.
- Engineered constructs often neglect homeostasis, impacting transplantation success.
- Understanding maturation is key for successful tissue engineering.
Purpose of the Study:
- To investigate the effect of encapsulation duration on engineered tissue maturation.
- To establish a protocol for evaluating critical conditions for tissue transplantation.
- To assess the viability and function of released cells post-encapsulation.
Main Methods:
- SHSY5Y human neuroblastoma cells encapsulated in alginate using electrohydrodynamic jetting for up to 4 weeks.
- In situ evaluation of extracellular matrix niche formation and tissue maturation via COL1A1 expression.
- In vitro assessment of cellular viability (apoptosis/necrosis, AlamarBlue) and functionality (immunocytochemistry) post-release.
Main Results:
- Cells encapsulated for 28 days met critical requirements for successful tissue transplantation.
- Engineered tissue homeostasis stabilization is essential for cell recovery.
- Maturation time significantly impacts the suitability of engineered tissues for transplantation.
Conclusions:
- Prolonged encapsulation (28 days) is necessary for SHSY5Y cell-laden constructs to achieve transplantation readiness.
- Accelerating maturation through growth factors or ECM molecules is a potential strategy.
- This study provides a protocol for assessing engineered tissue viability and maturation for transplantation.
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