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Dissecting antigen processing and presentation routes in dermal vaccination strategies
Anouk C M Platteel1, Sandrine Henri2, Dietmar M Zaiss3
1Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Vaccine
|October 29, 2017
Summary
Dermal DNA tattoo immunization effectively activates CD8+ T cells. Both bone marrow and host cells process antigens, while Langerhans cells are not essential for this immune response.
Area of Science:
- Immunology
- Vaccinology
Background:
- The skin offers accessible vaccination sites with immune cells.
- Antigen processing and presentation in dermal vaccination require further study.
Purpose of the Study:
- To determine antigen processing routes for CD8+ T cell activation after dermal DNA tattoo immunization.
- To investigate the roles of bone marrow-derived and host cells, including Langerhans cells, in this process.
Main Methods:
- Utilized a model antigen with an immunoproteasome-dependent epitope.
- Employed wild-type and gene-deficient mice lacking immunoproteasome subunits.
- Used bone marrow chimeric mice and depleted Langerhans cells.
Main Results:
- DNA tattoo immunization induced robust responses to the immunoproteasome-dependent epitope in wild-type mice.
- Mice lacking immunoproteasome subunits showed diminished responses.
- Both bone marrow-derived and host cells contributed to antigen processing.
- Langerhans cells were not critical for CD8+ T cell priming.
Conclusions:
- Dermal DNA tattoo immunization involves antigen processing by both bone marrow and non-bone marrow-derived cells.
- Langerhans cells do not play a primary role in antigen processing and T cell priming for this vaccination method.
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