Related Experiment Video
Updated: Feb 19, 2026

Comparing the Affinity of GTPase-binding Proteins using Competition Assays
Published on: October 8, 2015
G-Protein Gα13 Functions with Abl Kinase to Regulate Actin Cytoskeletal Reorganization
Limin Wang1, Dawei Wang1, Bowen Xing1
1Department of Physiology and Biophysics, Cornell University, Weill Cornell Medical College, NY, New York 10065, United States.
Abstract:
Heterotrimeric G-proteins are essential cellular signal transducers. One of the G-proteins, Gα13, is critical for actin cytoskeletal reorganization, cell migration, cell proliferation, and apoptosis. Previously, we have shown that Gα13 is essential for both G-protein-coupled receptor and receptor tyrosine kinase-induced actin cytoskeletal reorganization such as dynamic dorsal ruffle turnover and cell migration. However, the mechanism by which Gα13 signals to actin cytoskeletal reorganization is not completely understood. Here we show that Gα13 directly interacts with Abl tyrosine kinase, which is a critical regulator of actin cytoskeleton. This interaction is critical for Gα13-induced dorsal ruffle turnover, endothelial cell remodeling, and cell migration. Our data uncover a new molecular signaling pathway by which Gα13 controls actin cytoskeletal reorganization.
More Related Videos
07:41A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
10:27Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
Related Concept Videos
Activation and Inactivation of G Proteins
GTPases and their Regulation
Large G-proteins,...
GTPases and their Regulation
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Amplifying Signals via Enzymatic Cascade
GPCRs Regulate Adenylyl Cylase Activity