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A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Single-shot dual-wavelength interferometric microscopy.

Poorya Hosseini¹, Di Jin¹, Zahid Yaqoob²

¹Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.

Methods (San Diego, Calif.)

|October 29, 2017

Summary

This summary is machine-generated.

This study introduces a novel interferometric microscopy technique for simultaneous two-wavelength measurements. This method enhances cellular biophysical measurements without compromising resolution or requiring expensive equipment.

Keywords:

Cellular imaging Dispersion Interferometric microscopy Label-free imaging Quantitative phase

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Area of Science:

Biophysics
Optical Microscopy
Spectroscopy

Background:

Interferometric microscopy (IM) offers high-resolution imaging of biological samples with minimal photodamage.
Wavelength-dependent measurements in IM are crucial for refractometry and cellular biophysics.
Existing multi-wavelength IM methods often sacrifice temporal resolution, field of view, stability, or require costly components.

Purpose of the Study:

To present a novel, simple interferometric microscopy design for single-shot, dual-wavelength measurements.
To overcome the limitations of conventional multi-wavelength IM techniques.
To enable accurate, simultaneous quantification of wavelength-dependent optical properties.

Main Methods:

Developed a novel interferometric microscope design.
Employed single-shot, off-axis interferometric measurements.
Encoded dual-wavelength spectral images onto orthogonal spatial frequencies for Fourier domain separation.

Main Results:

Achieved clean separation of spectral information in Fourier space without resolution loss.
Demonstrated accurate, simultaneous quantification of polystyrene bead refractive indices at two distinct wavelengths.
Validated the efficacy of the novel encoding strategy for dual-wavelength analysis.

Conclusions:

The presented IM design offers a simple and effective solution for simultaneous dual-wavelength measurements.
This technique avoids the typical trade-offs associated with multi-wavelength interferometry.
The method holds potential for advancing cellular biophysical measurements and material characterization.