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Simultaneously extracting multiple parameters via multi-distance and multi-exposure diffuse speckle contrast

Jialin Liu1, Hongchao Zhang1, Jian Lu1

  • 1School of Science, Nanjing University of Science and Technology, No.200 Xiaolingwei Street, Nanjing 210094, China.

Biomedical Optics Express
|October 31, 2017
PubMed
Summary

Multi-distance and multi-exposure diffuse speckle contrast analysis (MDME-DSCA) noninvasively measures deep tissue microvasculature blood flow and optical properties. This advanced technique simultaneously extracts multiple parameters, unlike simpler methods.

Keywords:
(170.3660) Light propagation in tissues(170.6480) Spectroscopy, speckle(290.4210) Multiple scattering(300.1030) Absorption

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Physiological Measurement

Background:

  • Diffuse speckle contrast analysis (DSCA) enables noninvasive assessment of deep tissue microvasculature blood flow.
  • Temporal fluctuations in speckle patterns are influenced by blood flow index (αD), optical absorption (μ), reduced scattering coefficients (μs'), and coherence factor (β).
  • Existing methods face limitations in simultaneously extracting multiple physiological and optical parameters.

Purpose of the Study:

  • To develop and validate a multi-distance and multi-exposure diffuse speckle contrast analysis (MDME-DSCA) technique.
  • To enable simultaneous, noninvasive extraction of multiple microvasculature and optical parameters from deep tissues.
  • To compare the performance of MDME-DSCA against multi-exposure DSCA (ME-DSCA).

Main Methods:

  • Implementation of multi-distance and multi-exposure diffuse speckle contrast analysis (MDME-DSCA).
  • Simultaneous extraction of blood flow index (αD), optical absorption (μ), reduced scattering coefficients (μs'), and coherence factor (β).
  • Validation using simulated data and phantom experiments.

Main Results:

  • MDME-DSCA successfully validated for simultaneous extraction of multiple parameters (μ, μs', αD, β).
  • Demonstrated the impracticality of simultaneously obtaining multiple parameters using only multi-exposure DSCA (ME-DSCA).
  • Confirmed the capability of MDME-DSCA for noninvasive deep tissue microvasculature assessment.

Conclusions:

  • MDME-DSCA is a robust method for simultaneously quantifying multiple microvasculature and optical properties noninvasively.
  • The developed technique overcomes limitations of previous DSCA methods, offering more comprehensive physiological information.
  • This advancement holds potential for improved diagnostics and monitoring of deep tissue perfusion.