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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.5K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Capillary Electrophoresis: Instrumentation01:20

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Electrophoresis: Overview01:20

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Updated: Feb 19, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Dielectrophoretic microbead sorting using modular electrode design and capillary-driven microfluidics.

Jaione Tirapu-Azpiroz1, Yuksel Temiz2, Emmanuel Delamarche2

  • 1IBM Research-Brasil, Av. Pasteur 138&146, Rio de Janeiro, RJ, 22290-240, Brazil. jaionet@br.ibm.com.

Biomedical Microdevices
|October 31, 2017
PubMed
Summary
This summary is machine-generated.

This study presents a new microfluidic method for separating microbeads by size using dielectrophoresis. The compact, adaptive system efficiently sorts beads on portable chips with high accuracy.

Keywords:
DielectrophoresisLab on a chipMicrobeadsMicrofluidicParticle sorting

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Biophysics

Background:

  • Multiplexing assays require precise particle sorting based on physical properties.
  • Existing microbead separation methods in microfluidics can lack flexibility and throughput.
  • Dielectrophoresis (DEP) offers a label-free method for manipulating particles in microchannels.

Purpose of the Study:

  • To develop a continuous, compact, and adaptive microfluidic system for separating microbeads by size.
  • To optimize electrode layout and voltage configuration for efficient size-based separation using DEP.
  • To demonstrate the system's performance on portable, capillary-driven microfluidic chips.

Main Methods:

  • Simulated hydrodynamic drag and dielectrophoresis forces on polystyrene beads.
  • Optimized a novel electrode layout with slanted planar electrodes for alternating sorting and concentration.
  • Experimental validation using 10 μm and 5 μm beads on microfluidic chips.

Main Results:

  • Achieved efficient separation of 10 μm and 5 μm microbeads.
  • ~98% of concentrated beads were sorted into distinct streams.
  • Demonstrated high purity with only ~2% of 5 μm beads leaking into the 10 μm stream.

Conclusions:

  • The developed dielectrophoresis-based method enables efficient and continuous size-based separation of microbeads.
  • The compact, modular, and adaptive system is suitable for high-throughput multiplexing assays.
  • Implementation on capillary-driven microfluidic chips enhances portability and ease of use for various applications.