Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual uncertainty and task demands shape active sensing strategies in mice.

bioRxiv : the preprint server for biology·2026
Same author

Visual uncertainty and task demands shape active sensing strategies in mice.

Current biology : CB·2026
Same author

The Simons Collaboration on Ecological Neuroscience: Studying how the brain interacts with the world.

Neuron·2026
Same author

Visualizing and sonifying neurodata (ViSoND) for enhanced observation.

bioRxiv : the preprint server for biology·2026
Same author

Vision guides directed orienting movements during obstacle avoidance in mice.

bioRxiv : the preprint server for biology·2026
Same author

Ecological Visual Processing in the Mouse.

Annual review of neuroscience·2026
Same journal

Complex three-dimensional rearing environments amplify compensatory plasticity following early blindness.

eNeuro·2026
Same journal

c-Maf deletion in cortical somatostatin, but not parvalbumin, interneurons leads to absence-like epileptiform activity in mice.

eNeuro·2026
Same journal

Functional Characterization of Dopamine Projections Across Nucleus Accumbens Subregions in Reward-related, Anxiety-like and Locomotor Behaviors.

eNeuro·2026
Same journal

Translational profiling of Drd2-expressing populations reveals molecular heterogeneity of dentate gyrus mossy cells along the dorsoventral axis.

eNeuro·2026
Same journal

Movement Disorder Patients with Depression have Altered Corticostriatal Alpha-Beta Power Response to Reward and Loss.

eNeuro·2026
Same journal

Ocular speech tracking persists in blindness, but its dynamics and oculo-cerebral connectivity depend on visual status.

eNeuro·2026
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

3.4K

TU-Tagging: A Method for Identifying Layer-Enriched Neuronal Genes in Developing Mouse Visual Cortex.

Johanna Tomorsky1,2, Leah DeBlander1,2, Clifford G Kentros1,2,3,4

  • 1Institute of Neuroscience, University of Oregon, Eugene, OR 97403.

Eneuro
|November 1, 2017
PubMed
Summary
This summary is machine-generated.

Thiouracil (TU)-tagging can now profile specific neuronal RNAs in the mouse brain. This method successfully labels RNA in vivo, demonstrating 4TU crosses the blood-brain barrier.

Keywords:
TU-tagginggene expressionlayer 2/3postnataltranscriptomicsvisual cortex

More Related Videos

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging
06:18

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Published on: November 21, 2023

1.4K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

14.1K

Related Experiment Videos

Last Updated: Feb 19, 2026

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

3.4K
Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging
06:18

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Published on: November 21, 2023

1.4K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

14.1K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Thiouracil (TU)-tagging enables cell type-specific RNA labeling via uracil phosphoribosyl transferase (UPRT) and 4TU.
  • Previous applications focused on endothelial cells; its utility in the brain, particularly crossing the blood-brain barrier (BBB), was unknown.

Purpose of the Study:

  • To test if 4TU can cross the BBB and if Cre-dependent UPRT expression can purify neuronal RNAs in the mouse brain.
  • To identify genes specifically expressed in distinct visual cortex layers.

Main Methods:

  • Utilized a Cre-dependent UPRT transgene (CA>GFPstop>HA-UPRT) crossed with Sepw1-cre (layer 2/3) and Nr5a1-cre (layer 4) mouse lines.
  • Purified thiol-tagged mRNA from visual cortex of postnatal day 12 mice.
  • Compared RNA profiles from transgenic mice to wild-type controls.

Main Results:

  • Demonstrated successful purification of cell type-specific mRNA from the intact mouse brain.
  • Provided the first evidence that 4TU can cross the BBB and label RNA in vivo.
  • Identified genes enriched in layer 2/3 visual cortex by comparing Sepw1-purified RNA to controls.

Conclusions:

  • Cre-dependent UPRT expression is effective for purifying cell type-specific mRNA in the mouse brain.
  • 4TU is capable of crossing the BBB for in vivo RNA labeling.
  • This technique advances cell type-specific RNA profiling in the central nervous system.