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Solvatochromic dye LDS 798 as microviscosity and pH probe.
Hung Doan1, Marlius Castillo, Micheline Bejjani
1Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA. z.gryczynski@tcu.edu.
Physical Chemistry Chemical Physics : PCCP
|November 2, 2017
Summary
Styryl dyes like LDS 798 can detect viscosity and pH changes in cells, expanding their use beyond monitoring mitochondrial potential. These findings are crucial for accurate cellular sensing applications.
Area of Science:
- Biophysical chemistry
- Cellular imaging
- Fluorescent probes
Background:
- Styryl dyes, particularly LDS group dyes, are established solvatochromic and electrochromic probes.
- Their application is primarily for monitoring mitochondrial potential in cellular environments.
- The response of these dyes to viscosity, pH, and temperature fluctuations remains largely uncharacterized.
Purpose of the Study:
- To investigate the sensing capabilities of LDS 798 (Styryl-11) beyond polarity.
- To determine if LDS 798 can respond to environmental viscosity and pH changes.
- To assess the potential of LDS 798 as a multi-parametric cellular sensor.
Main Methods:
- Utilized fluorescence lifetime measurements to assess viscosity sensing.
- Employed ratiometric intensity changes to detect pH variations.
- Analyzed spectral shifts for probing polarity changes.
- Conducted experiments in controlled cellular environments.
Main Results:
- LDS 798 demonstrated sensitivity to environmental viscosity through fluorescence lifetime alterations.
- The dye exhibited ratiometric intensity changes in response to pH variations, independent of polarity.
- Spectral changes of LDS 798 were confirmed as indicators of polarity.
- The study established that LDS 798 can sense viscosity and pH.
Conclusions:
- LDS 798 is a versatile probe capable of sensing viscosity and pH in addition to polarity.
- Fluorescence lifetime and ratiometric intensity are key parameters for viscosity and pH detection, respectively.
- Comprehensive consideration of media properties is essential when using LDS 798 as a sensor in cellular studies.
