Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wave-optics simulation model for full-field modulation-based tensor tomography using a random wavefront modulator.

Optics express·2026
Same author

The lung in acute respiratory failure: insights from synchrotron radiation imaging.

Intensive care medicine experimental·2026
Same author

In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography.

Nature communications·2026
Same author

Characterizing heterogeneity and subphenotyping acute respiratory distress syndrome with computed tomography.

Intensive care medicine experimental·2026
Same author

FaXToR: the hard X-ray micro-tomography beamline at the Spanish synchrotron ALBA.

Journal of synchrotron radiation·2025
Same author

Nano-laminography with a transmission X-ray microscope.

Journal of synchrotron radiation·2025

Related Experiment Video

Updated: Feb 19, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.8K

Characterization of a sCMOS-based high-resolution imaging system.

Alberto Mittone1, Ilja Manakov2, Ludovic Broche3

  • 1European Synchrotron Radiation Facility, 71 Avenue des Martyrs, Grenoble 38000, France.

Journal of Synchrotron Radiation
|November 2, 2017
PubMed
Summary

This study analyzes a new sCMOS-based detection system for high-resolution computed-tomography imaging at the European Synchrotron Radiation Facility. The system offers fast imaging but involves trade-offs in image quality due to X-ray beam inhomogeneity.

Keywords:
X-ray imagingimage detectorimage quality

More Related Videos

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

8.1K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.5K

Related Experiment Videos

Last Updated: Feb 19, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.8K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

8.1K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.5K

Area of Science:

  • Medical Imaging
  • Synchrotron Radiation Technology
  • Detector Physics

Background:

  • High-resolution computed-tomography (CT) imaging is crucial for biomedical applications.
  • Advanced detection systems are essential for optimizing CT performance.
  • The European Synchrotron Radiation Facility (ESRF) ID17 beamline requires high-performance imaging detectors.

Purpose of the Study:

  • To evaluate the performance of a novel sCMOS-based detection system for high-resolution CT.
  • To characterize key imaging parameters of the PCO.Edge5.5 detector at the ID17 beamline.
  • To assess the suitability of the system for biomedical synchrotron imaging.

Main Methods:

  • Characterization of optical properties, linearity, and lag.
  • Modulation Transfer Function (MTF) and detective quantum efficiency (DQE) measurements.
  • Photon transfer curve analysis at 35 and 50 keV with 1x and 2x magnification.

Main Results:

  • The sCMOS system demonstrated good agreement with manufacturer specifications.
  • Measured pixel sizes were 3.1 µm (2x mag) and 6.2 µm (1x mag).
  • Fast imaging is achievable, though with some image quality compromises.

Conclusions:

  • The novel sCMOS detector is suitable for high-resolution CT at the ESRF ID17 beamline.
  • X-ray beam inhomogeneity was identified as a limiting factor for system performance.
  • The system offers a balance between imaging speed and quality for synchrotron-based CT applications.