Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mucin-derived sugars act as metabolic brakes controlling growth initiation in <i>Akkermansia muciniphila</i>.

Gut microbes·2026
Same author

A stationary phase-specific bacterial green light sensor for enhancing metabolite production.

Nature communications·2025
Same author

Probiotic acoustic biosensors for noninvasive imaging of gut inflammation.

Nature communications·2025
Same author

Changes in Spo0A~P pulsing frequency control biofilm matrix deactivation.

PLoS computational biology·2025
Same author

Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

A <i>Staphylococcus aureus</i> Virulence Inhibitor Identified by SaeRS Refactoring and Screening in <i>Bacillus subtilis</i>.

ACS synthetic biology·2025
Same journal

Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.

ACS synthetic biology·2026
Same journal

Heterologous Expression of an Abandoned Termite Mound Fungus Gene Cluster Reveals a Protective Aldehyde-Alcohol Cycle and a Candidate Termiticidal Metabolite.

ACS synthetic biology·2026
Same journal

A Framework for the In Vivo Production of Extensively Engineered Thiopeptides.

ACS synthetic biology·2026
Same journal

A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.

ACS synthetic biology·2026
Same journal

Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis.

ACS synthetic biology·2026
Same journal

Sequential Plasmid Curing and Genome Editing in <i>Escherichia coli</i> Nissle 1917.

ACS synthetic biology·2026
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

17.7K

Engineering an E. coli Near-Infrared Light Sensor.

Nicholas T Ong1, Evan J Olson1, Jeffrey J Tabor1

  • 1Department of Bioengineering, ‡Department of Biosciences, Rice University , 6100 Main Street, Houston, Texas 77005, United States.

ACS Synthetic Biology
|November 2, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel optogenetic tool using near-infrared (NIR) light to control gene expression in bacteria. This system, based on the BphP1-PpsR2 photoreceptor, offers precise and rapid control, advancing bacterial optogenetics.

Keywords:
bacteriophytochromenear-infrared lightoptogeneticssynthetic biology

More Related Videos

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
05:52

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Published on: June 28, 2018

12.2K
Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy
08:25

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

Published on: April 27, 2021

4.2K

Related Experiment Videos

Last Updated: Feb 19, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

17.7K
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
05:52

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Published on: June 28, 2018

12.2K
Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy
08:25

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

Published on: April 27, 2021

4.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Optogenetics enables precise control of biological processes using light and engineered photoreceptors.
  • Near-infrared (NIR) light is advantageous for optogenetics due to low phototoxicity and spectral properties.
  • Bacteriophytochrome photoreceptor 1 (BphP1) senses NIR light and regulates transcription by interacting with the PpsR2 repressor.

Purpose of the Study:

  • To engineer and characterize a novel bacterial optogenetic system utilizing NIR light.
  • To identify optimal promoter and repressor combinations for NIR-activated gene expression in E. coli.
  • To demonstrate the functionality and advantages of the BphP1-PpsR2 system for bacterial optogenetics.

Main Methods:

  • Screening of promoter libraries for repression by Rhodopseudomonas palustris PpsR2.
  • Engineering an Escherichia coli strain for biliverdin IXα chromophore production.
  • Optimizing expression of Rps. palustris bphP1 and ppsR2 for NIR-activated transcription.

Main Results:

  • Identified Bradyrhizobium sp. BTAi1 crtE as the most strongly repressed promoter in E. coli.
  • Successfully demonstrated NIR-activated transcription using the optimized BphP1-PpsR2 system.
  • The developed system exhibits rapid response dynamics and is the most red-shifted bacterial optogenetic tool reported to date.

Conclusions:

  • The BphP1-PpsR2 system provides a powerful new tool for NIR-activated bacterial optogenetics.
  • This system offers advantages over existing tools, including no requirement for a second messenger and rapid response.
  • The red-shifted nature of this tool expands the possibilities for multiplexing and in vivo applications in bacteria.