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Related Experiment Video

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Comparing Objective Conjunctival Hyperemia Grading and the Ocular Surface Disease Index Score in Dry Eye Syndrome During COVID-19
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Detecting Change in Conjunctival Hyperemia Using a Pixel Densitometry Index.

Vito Romano1, Bernhard Steger2, Matthias Brunner1

  • 1a Department of Corneal and External Eye Diseases, St. Paul's Eye Unit , The Royal Liverpool University Hospital , Liverpool , UK.

Ocular Immunology and Inflammation
|November 3, 2017
PubMed
Summary

A new pixel densitometry index (PDI) objectively measures ocular surface inflammation (OSI) changes. This method shows greater consistency than traditional grading for conjunctival hyperemia.

Keywords:
Angiographyconjunctival inflammationmonitoringpixel densitometry

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Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Inflammation Research

Background:

  • Ocular surface inflammation (OSI) assessment often relies on subjective grading.
  • Objective and quantifiable methods are needed for accurate OSI evaluation.

Purpose of the Study:

  • To investigate a novel pixel densitometry index (PDI) for objective measurement of ocular surface inflammation (OSI).
  • To compare the reliability of PDI with traditional Efron's grading for conjunctival hyperemia.

Main Methods:

  • Two independent observers graded conjunctival hyperemia using Efron's scale.
  • Color photographs and indocyanine green angiography were obtained before and after phenylephrine (PE) instillation.
  • Pixel densitometry index (PDI) was calculated and compared with Efron's grades.

Main Results:

  • The PDI demonstrated a statistically significant reduction post-PE in both inflamed and non-inflamed eyes (p=0.036 and p=0.0024, respectively).
  • A 25% reduction in Efron grades was observed post-PE (p < 0.01).
  • The PDI exhibited a lower coefficient of variation (42-50%) compared to Efron's grades (59-72%), indicating higher reliability.

Conclusions:

  • The pixel densitometry index (PDI) offers an objective method for detecting changes in conjunctival hyperemia.
  • PDI shows potential for noninvasive angiography applications, including optical coherence tomography-based angiography.