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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
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Modern methods for laboratory diversification of biomolecules
Sinisa Bratulic1, Ahmed H Badran1
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Current Opinion in Chemical Biology
|November 3, 2017
Summary
Scientists are developing new mutagenesis strategies to create genetic diversity for protein evolution and genome engineering. These methods allow precise DNA modifications for research and potential therapeutic applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Genetic variation is essential for evolution, but low spontaneous mutation rates limit exploration of sequence space.
- Efficient and targeted nucleic acid diversification is crucial for protein evolution and genome engineering.
Purpose of the Study:
- To review current mutagenesis strategies for directed evolution and genome engineering.
- To highlight advancements enabling precise genetic modifications.
Main Methods:
- Review of existing literature on mutagenesis techniques.
- Analysis of methods for generating genetic diversity in biomolecules and genomes.
Main Results:
- A comprehensive overview of state-of-the-art mutagenesis strategies is presented.
- These methods facilitate modifications of genes, pathways, and whole genomes.
Conclusions:
- Advanced mutagenesis tools offer facile improvements for basic and applied research.
- These strategies are paving the way for genome editing therapeutics.
