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Particle aggregates formed during furfuryl methacrylate plasma polymerization affect human mesenchymal stem cell
Hanieh Safizadeh Shirazi1, Nicholas Rogers1, Andrew Michelmore2
1Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, SA 5095, Australia; Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Mawson Lakes, SA 5095, Australia.
Colloids and Surfaces. B, Biointerfaces
|November 3, 2017
Summary
Plasma polymerized Furfuryl Methacrylate (ppFMA) thin films support human mesenchymal stem cell (hMSC) expansion. Uniform ppFMA surfaces promote hMSC attachment and growth, unlike those with particle aggregates.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Surface Chemistry
Background:
- Human mesenchymal stem cells (hMSCs) are crucial for regenerative medicine.
- Ex vivo expansion of hMSCs is essential for therapeutic applications.
- Substrate-independent thin films offer potential for scalable hMSC culture.
Purpose of the Study:
- To evaluate plasma polymerized Furfuryl Methacrylate (ppFMA) surfaces for hMSC culture.
- To investigate the impact of ppFMA surface aggregates on hMSC attachment and proliferation.
- To assess the suitability of ppFMA for large-scale hMSC expansion.
Main Methods:
- hMSCs were cultured on ppFMA surfaces with and without particle aggregates.
- Metabolic and DNA quantification assays assessed cell attachment and growth.
- Phenotypic analysis and time-lapse imaging evaluated hMSC behavior and morphology.
Main Results:
- Uniform ppFMA surfaces supported hMSC attachment and growth comparable to Thermanox.
- Surfaces with particle aggregates exhibited reduced hMSC attachment and slower growth.
- Particle aggregates disrupted hMSC attachment by creating a weak boundary layer.
- hMSC phenotype remained stable on uniform ppFMA surfaces after 7 and 14 days.
Conclusions:
- Uniform ppFMA surfaces are suitable for hMSC culture and expansion.
- Particle aggregates on ppFMA surfaces negatively impact hMSC attachment and growth.
- ppFMA offers a promising substrate-independent interface for scalable hMSC expansion in bioreactors or scaffolds.
