Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro core particle and nucleosome assembly at physiological ionic strength.

A Ruiz-Carrillo, J L Jorcano, G Eder

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1979
    PubMed
    Summary

    Researchers efficiently assembled nucleosome core particles in vitro using histones and DNA. The core histone octamer organizes approximately 167 base pairs of DNA, suggesting a potential in vivo assembly mechanism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [DNA ejection from single phage particles].

    Virologie (Montrouge, France)·2023
    Same author

    Erratum: Publisher's Note: "Skin-on-a-chip models: General overview and future perspectives" [APL Bioengineering <b>5</b>, 030901 (2021)].

    APL bioengineering·2021
    Same author

    Skin-on-a-chip models: General overview and future perspectives.

    APL bioengineering·2021
    Same author

    A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept.

    Scientific reports·2021
    Same author

    Cloning and characterization of keratin D, a murine endodermal cytoskeletal protein induced during in vitro differentiation of F9 teratocarcinoma cells.

    Roux's archives of developmental biology : the official organ of the EDBO·2017
    Same author

    [The anterolateral thigh flap: its versatility in oncological soft tissue reconstruction of the head and neck region].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2011

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Chromatin Structure

    Background:

    • Nucleosomes are fundamental units of DNA packaging in eukaryotes.
    • Understanding nucleosome assembly is crucial for gene regulation and DNA replication.

    Purpose of the Study:

    • To investigate the in vitro assembly of nucleosome core particles.
    • To determine the DNA length organized by the core histone octamer.
    • To explore the potential in vivo assembly mechanisms.

    Main Methods:

    • In vitro reconstitution of nucleosome core particles using purified histones and DNA.
    • Assays including DNase digestion, DNA supercoiling, and electron microscopy.
    • Analysis of DNA length protected by histone octamers and histone H1.

    Related Experiment Videos

    Main Results:

    • Efficient nucleosome core particle assembly achieved at physiological ionic strength.
    • Demonstrated heterogeneity in core particle spacing on DNA.
    • Core histone octamer organizes approximately 167 base pairs of DNA, with 20 base pairs rapidly digested.
    • Histone H1 binding protects about 165 base pairs from nuclease digestion.

    Conclusions:

    • Nucleosome assembly can occur via simultaneous or sequential addition of histone complexes.
    • The findings suggest a model for in vivo nucleosome assembly involving H3.H4 tetramers and H2A.H2B dimers.
    • The core histone octamer's DNA organization capacity is larger than previously estimated for core particles.