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Updated: Feb 19, 2026
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FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
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Adipose Stem Cells Incorporated in Fibrin Clot Modulate Expression of Growth Factors
Kelsy R Siegel1, Tracy N Clevenger2, Dennis O Clegg2
1Center for Stem Cell Biology and Engineering, Neuroscience Research Institute, and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, California, U.S.A..
Summary
Whole blood fibrin clots effectively capture platelets and release essential growth factors. Incorporating adipose-derived stem cells (ASCs) modulates these factors, suggesting potential for future stem cell therapies.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Stem Cell Biology
Background:
- Fibrin clots derived from whole blood are being explored as potential scaffolds.
- Understanding growth factor release from these clots is crucial for therapeutic applications.
- The role of adipose-derived stem cells (ASCs) in modulating clot properties is under investigation.
Purpose of the Study:
- To assess platelet capture by whole blood fibrin clots.
- To evaluate the expression, secretion, and retention of VEGF, PDGF, and bFGF from fibrin clots.
- To determine how allogeneic ASCs influence these growth factor levels.
Main Methods:
- Human whole blood was used to form fibrin clots in a specialized device.
- In vitro experiments compared clots with and without ASCs, alongside a control group.
- Enzyme-linked immunosorbent assay and immunohistochemistry were used to analyze growth factor secretion and retention.
Main Results:
- Fibrin clots captured approximately 98% of available platelets.
- ASC-incorporated clots showed significantly higher VEGF secretion compared to controls.
- ASC incorporation modulated PDGF and bFGF levels and immunoreactivity.
Conclusions:
- Fibrin clots effectively retain high platelet concentrations.
- Incorporation of ASCs modulates the secretion and immunoreactivity of key growth factors (VEGF, PDGF, bFGF).
- These findings support the potential of whole blood fibrin clots as scaffolds for stem cell therapies.
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