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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
Determination and optimization of a strong promoter element from Bacillus amyloliquefaciens by using a promoter probe
Yuling Liao1,2, Bin Wang1, Yanrui Ye3,4
1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, Guangdong, People's Republic of China.
Objective:
To construct a promoter probe vector, pBE-bgaB, to screen strong promoters from Bacillus amyloliquefaciens.
Results:
266 colonies containing active promoter elements from the genomic DNA of B. amyloliquefaciens were identified. Among these, promoter P41 exhibited the strongest β-Gal activity in Escherichia coli and B. amyloliquefaciens. Sequence analysis showed that promoter P41 contained P ykuN , a ykuN gene encoding flavodoxin. Optimization of the ribosome-binding site from P41 to P382 improved β-Gal activity by ~ 200%.
Conclusion:
A new strong promoter for protein expression and genetic engineering of Bacillus species.
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