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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Feb 19, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Multiplexed Nucleic Acid Programmable Protein Arrays.

Xiaobo Yu¹, Lusheng Song², Brianne Petritis²

¹State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (PHOENIX Center, Beijing), Beijing Institute of Radiation Medicine, Beijing, 102206, China.

|November 8, 2017

Summary

This summary is machine-generated.

Multiplexed Nucleic Acid Programmable Protein Arrays (M-NAPPA) enhance protein microarray density by synthesizing multiple proteins per spot. This cost-effective method facilitates high-throughput translational research and antigen discovery.

Keywords:

Antibody Biomarker.Cell-free protein microarray Protein-protein interaction Proteomics

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Last Updated: Feb 19, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Biotechnology
Molecular Biology
Immunology

Background:

Cell-free protein microarrays are valuable tools for research, enabling in situ synthesis of naturally-folded proteins.
Protein diffusion during expression limits feature density in traditional arrays, hindering high-throughput applications.

Purpose of the Study:

To develop a multiplexed protein array platform (M-NAPPA) to overcome feature density limitations.
To demonstrate the efficacy of M-NAPPA for detecting protein interactions and identifying disease-related antigens.

Main Methods:

Developed Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA) technology.
Multiplexed up to five gene plasmids within a single spot for simultaneous protein expression.
Combined M-NAPPA with photolithography-based silicon nano-well platform for ultra-high density arrays.
Utilized protein-specific antibodies for detection and ELISA for validation.

Main Results:

M-NAPPA successfully displayed and detected multiple proteins of varying sizes within the same feature.
Comparable detection of protein-protein interactions and serological antibody targets were achieved with M-NAPPA and non-multiplexed arrays.
An ultra-high density microarray displaying over 16,000 proteins was fabricated.
Identified four novel tuberculosis-related antigens in BCG-vaccinated guinea pigs.

Conclusions:

Multiplexing features on protein microarrays is a cost-effective fabrication approach.
M-NAPPA technology significantly enhances protein microarray feature density.
This platform holds potential for high-throughput translational research and biomarker discovery.