Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combined Lingzhi Huang capsules and Zeng Jian health tonic accelerates skin wound healing via BMP5-mediated inhibition of ferroptosis.

Frontiers in immunology·2026

Same author

Mechanisms of therapy resistance in the tumor microenvironment: Insights from antibody array-based cytokine profiling.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2026

Same author

Strategies for Monitoring Serum Protein Degradation With an Antibody Array-Based Technology.

Journal of clinical laboratory analysis·2026

Same author

A Novel Flow Cytometry Array for High Throughput Detection of SARS-CoV-2 Antibodies.

Vaccines·2025

Same author

Combination detection of IgG- and IgA-related autoantibodies for the early diagnosis of gastric cancer.

Cancer biomarkers : section A of Disease markers·2025

Same author

Unraveling tumor cell‑tumor microenvironment crosstalk through antibody array technologies (Review).

International journal of oncology·2025

Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 28, 2025

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Sandwich-Based Antibody Arrays for Protein Detection.

Ying Qing Mao¹, Brianne Petritis², Ruo-Pan Huang^2,3,4,5

¹RayBiotech Life, Peachtree Corners, GA, USA. mike@raybiotech.com.

Methods in Molecular Biology (Clifton, N.J.)

|November 25, 2020

Summary

This summary is machine-generated.

This study introduces a rapid, 1-day method for processing samples using quantitative sandwich-based antibody arrays. This fluorescence-based technique allows for simultaneous detection of multiple proteins, offering a cost-effective alternative to traditional single-protein assays.

Keywords:

Antibodies Antibody array Array Fluorescence Immunoassay Quantitative array Sandwich immunoassay

More Related Videos

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Related Experiment Videos

Last Updated: Nov 28, 2025

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Area of Science:

Biochemistry
Immunotechnology
Proteomics

Background:

Sandwich-based antibody arrays allow for multiplexed protein detection.
These arrays offer a time- and cost-effective alternative to single-plex assays.
The "sandwich" technique involves a capture antibody and a detection antibody.

Purpose of the Study:

To describe a streamlined 1-day procedure for sample processing.
To utilize quantitative sandwich-based antibody arrays on a glass substrate.
To enable fluorescence-based detection of multiple proteins.

Main Methods:

Development of a 1-day protocol for sample processing.
Application of quantitative sandwich-based antibody arrays.
Utilizing fluorescence for protein detection on a glass substrate.
Processing of biological samples like serum and cell lysates.

Main Results:

Successful implementation of a 1-day procedure.
Demonstration of multiplexed protein detection capabilities.
Quantitative analysis of proteins in serum and cell lysates.

Conclusions:

The described method provides an efficient and cost-effective approach for multiplexed protein analysis.
This 1-day protocol facilitates rapid biomarker discovery and validation.
The fluorescence-based antibody array platform is suitable for various biological samples.