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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Paperfluidic Chip Device for Small RNA Extraction, Amplification, and Multiplexed Analysis.

Huaping Deng¹, Xiaoming Zhou¹, Qianwen Liu²

¹MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.

ACS Applied Materials & Interfaces

|November 9, 2017

Summary

This study introduces a novel paper-based device for sensitive, multiplexed small RNA detection. This point-of-care assay offers a promising tool for disease biomarker analysis.

Keywords:

in situ amplification multiplexed detection paperfluidic chip quantum dots small RNA analysis

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Last Updated: Feb 19, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

Microfluidic Applications for Disposable Diagnostics

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Microfluidic Chip Fabrication and Method to Detect Influenza

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Published on: March 26, 2013

Area of Science:

Biomarker Discovery
Analytical Chemistry
Point-of-Care Diagnostics

Background:

Small RNAs are crucial biomarkers for various diseases.
Sensitive and multiplexed detection methods are needed for point-of-care (POC) applications.
Existing methods often require complex laboratory equipment.

Purpose of the Study:

To develop an integrated paperfluidic chip for multiplexed small RNA analysis.
To create a portable and user-friendly device for rapid disease biomarker detection.
To demonstrate the clinical applicability of the developed assay.

Main Methods:

Developed an integrated paperfluidic chip using poly(ether sulfone) (PES) for small RNA extraction and purification.
Employed a hairpin probe-exponential amplification reaction (HP-EXPAR) for signal amplification.
Utilized quantum dots (QDs) as signal labels and magnetic sheets for multilayer assembly.
Designed a foldable, multilayer chip for simultaneous multiplexed detection.

Main Results:

Achieved a sensitivity range from 3 × 10^5 to 3 × 10^8 copies, with a limit of detection at 3 × 10^6 copies.
Demonstrated accurate multiplex small RNA analysis from cancer cells, comparable to qRT-PCR.
Successfully analyzed miRNAs from clinical tumor samples, validating clinical applicability.

Conclusions:

The developed paper-based device enables sensitive, multiplexed small RNA detection.
The integrated system is suitable for point-of-care applications.
This technology holds significant promise for disease diagnosis and biomarker monitoring.