MiR-20a-5p represses the multi-drug resistance of osteosarcoma by targeting the SDC2 gene

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A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
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Fangfang Zhao1, Youguang Pu1, Mingda Cui2

  • 1Cancer Epigenetics Program, Anhui Cancer Hospital, West Branch of Anhui Provincial Hospital, Anhui Medical University, Hefei, 230031 Anhui China.

Cancer Cell International
|November 10, 2017
PubMed
Abstract

Insights

MicroRNA-20a-5p (miR-20a-5p) levels are lower in chemoresistant osteosarcoma (OS) cells. Upregulating miR-20a-5p combats OS chemoresistance by targeting syndecan 2 (SDC2).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Chemoresistance is a major challenge in osteosarcoma (OS) treatment.
  • MicroRNAs (miRNAs) play crucial roles in regulating gene expression and cancer chemoresistance.

Purpose of the Study:

  • To investigate the role of miR-20a-5p in osteosarcoma chemoresistance.
  • To identify the downstream targets of miR-20a-5p involved in OS chemoresistance.

Main Methods:

  • RNA-sequencing based miR-omic analysis of chemo-sensitive and resistant OS cells.
  • Validation of miR-20a-5p and its target gene syndecan 2 (SDC2) expression at mRNA and protein levels.
  • Functional studies using miRNA mimics/antagomirs and SDC2 siRNA/overexpression to assess chemoresistance.

Main Results:

  • miR-20a-5p expression was significantly lower in chemoresistant OS cells (SJSA-1) compared to chemo-sensitive cells (G-292).
  • Overexpression of miR-20a-5p reduced chemoresistance in OS cells in vitro and in vivo.
  • SDC2 was identified as a direct target of miR-20a-5p, mediating its effect on OS chemoresistance.

Conclusions:

  • The miR-20a-5p/SDC2 axis is implicated in regulating osteosarcoma chemoresistance.
  • miR-20a-5p and SDC2 represent potential diagnostic biomarkers and therapeutic targets for OS patients.
Keywords:
ChemoresistanceOsteosarcomaSDC2miR-20a-5p

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